| Cryo

There are 10 total main units in the Cryo category. Please use the checkboxes to filter the results using the menu to the left.

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ACE900 Freeze Fracture System

A breakthrough in sample preparation yield, simplicity & speed in high-end cryo coating. Vitrified frozen structures are extremely sensitive to environmental influences and need to be protected from the formation of artefacts. Prepare your samples for detailed image evaluation under optimal conditions:

  • Cold shield around the sample avoids water molecules freezing onto your sample
  • Accurate temperature control during the complete process
  • Clean Knife - fresh blade for each cut avoids contamination
  • Instant, fast e-beam coating, which captures detailed surface information
  • Protected vacuum transfer to other analysis systems

ACE900 Freeze Fracture System

A breakthrough in sample preparation yield, simplicity & speed in high-end cryo coating. Vitrified frozen structures are extremely sensitive to environmental influences and need to be protected from the formation of artefacts. Prepare your samples for detailed image evaluation under optimal conditions:

  • Cold shield around the sample avoids water molecules freezing onto your sample
  • Accurate temperature control during the complete process
  • Clean Knife - fresh blade for each cut avoids contamination
  • Instant, fast e-beam coating, which captures detailed surface information
  • Protected vacuum transfer to other analysis systems

ACE900 Freeze Fracture System

A breakthrough in sample preparation yield, simplicity & speed in high-end cryo coating. Vitrified frozen structures are extremely sensitive to environmental influences and need to be protected from the formation of artefacts. Prepare your samples for detailed image evaluation under optimal conditions:

  • Cold shield around the sample avoids water molecules freezing onto your sample
  • Accurate temperature control during the complete process
  • Clean Knife - fresh blade for each cut avoids contamination
  • Instant, fast e-beam coating, which captures detailed surface information
  • Protected vacuum transfer to other analysis systems

ACE900 Freeze Fracture System

A breakthrough in sample preparation yield, simplicity & speed in high-end cryo coating. Vitrified frozen structures are extremely sensitive to environmental influences and need to be protected from the formation of artefacts. Prepare your samples for detailed image evaluation under optimal conditions:

  • Cold shield around the sample avoids water molecules freezing onto your sample
  • Accurate temperature control during the complete process
  • Clean Knife - fresh blade for each cut avoids contamination
  • Instant, fast e-beam coating, which captures detailed surface information
  • Protected vacuum transfer to other analysis systems

AFS2 Automatic Freeze Substitution System

The Leica EM AFS2 performs freeze substitution and progressive lowering of temperature (PLT) techniques and allows low temperature embedding and polymerization of resins.

The Leica EM FSP (freeze substitution processor), an automatic reagent handling system combined with the Leica EM AFS2, dispenses reagents for both freeze substitution and PLT applications. The LED illumination from within the chamber and the attached stereomicroscope for viewing and positioning of samples ensures ease of use.

BAF060 Freeze Fracture System

The Leica BAF060 is a high-end sample preparation system for freeze fracturing, etching, and freeze drying of double replica, high resolution carbon/metal mix coatings for TEM and SEM analysis.

Using the Leica VCT100 in conjunction, conduct specimen replication by electron beam evaporation, double layer coating of specimens for cryo SEM analysis and cryo coating for cryo SEM.

The Leica EM BAF060 features an advanced microtome, flexible shadowing options with two independent electron beam sources and a load-lock transfer system.

CryoCLEM Cryo Correlative Light and Electron Microscopy

Correlative Light and Electron Microscopy

Researchers can only optimise results when their samples are well-preserved during the entire process from sample preparation to imaging. To enable you to focus on what really matters, Leica Microsystems has developed a solution for the complete workflow from cryo fixation to cryo fluorescence light microscopy. This unique solution offers premium instrumentation from electron microscopy sample preparation to fluorescence light microscopy, including analysis capabilities with the Leica Application Suite microscopy software platform.

GP Automatic Plunge Freezing

For the preparation of vitrified fluid samples or extremely thin samples for cryo-TEM, including biological suspensions and industrial emulsions in both, aqueous and inorganic solvents.

The Leica GP plunge freezes samples into liquid ethane after removing excess fluid by automatic blotting. This is the bare grid technique. Prior to freezing the sample is maintained in a temperature and humidity controlled environmental chamber, adjustable between +4°C and +60°C and room humidity to 99%.

The GP can be used to plunge freeze samples not only on EM grids, but also sapphire discs and samples in freeze fracture planchettes.

ICE High Pressure Freezer

High pressure freezing captures the intricate changes in fine structure or cellular dynamics.

High pressure freezing combined with light stimulation is the platform for your discoveries.

  • Synchronize freezing and stimulation to the millisecond to capture the moment that interests you most
  • Capture and resolve highly dynamic processes at a nanometer scale and millisecond time precision
  • Cryo-immobilize your aqueous samples under high pressure and uncover the world's secrets

VCT100 Vacuum Cryo Transfer System

The Leica EM VCT100 is a contamination-free cryo transfer system which cross-links preparation units with various analysis systems via a transfer shuttle connected to a load-lock.

Samples are transferred in a well-defined environment (e.g. protective gas or high vacuum conditions) from a preparation instrument to the analysis unit. In addition, the specimen can be kept at low temperature for cryo techniques. The load-lock can mount to any vacuum operated SEM, FIB, SIMS, AFM or XPS chamber.

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