| Nikon Imaging | DS-Qi2 Monochrome Cooled CMOS

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Product Summary

For the first time ever, Nikon has equipped with a Nikon digital SLR camera FX-format CMOS sensor and optimized it for microscopy. The result is an ultra-high quality 16.25 megapixel monochrome camera that features high pixel density, high sensitivity and low noise, making it an excellent choice for applications in quantitative fluorescence imaging.


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Features

Detects even faint fluorescent signals

7.3μm pixels, high quantum efficiency, and very low read noise allow the DS-Qi2 to read in even faint fluorescent signals.


Reliable quantitative analysis made possible

With a linearity error of ±1%, the DS-Qi2 is a superb tool for measuring intensities in fluorescence samples, including timebases intensity measurement and radiometric measurement.

Fast focusing, even with fluorescent images

With a high-sensitivity CMOS sensor and USB 3.0-based data transfer, the DS-Qi2 enables high-speed live imaging and image capture at up to 45 fps (1636x1088 pixels).

Acquires dim fluorescent signals with ultra-low noise

2.2 electrons read noise coupled with a large full-well capacity allows the acquisition of fluorescence images with very little noise and very high dynamic range.


LLC-PK1 cells expressing GFP-EB3 tubulin with low noise. Large linear full well capacity allows acquiring both the brightest and dimmest areas in a single capture. Sample courtesy of: Michael Davidson, National High Magnetic Field Laboratory, Florida State University

Fluorescent time-lapse imaging through integration with NIS-Elements software

With a large field of view and pixel density, and low noise, the DS-Qi2 is ideal for time-resolved imaging applications.


Time-lapse images (every 1 second) of LLC-PK1 cells with GFP-EB3 tubulin. Each image represents the maximum intensity projection of the time-lapse, allowing visualization of the end-binding protein located on the microtubule plus-ends, and allowing tracing of the microtubule path. DS-Qi2 captures an extremely large field of view, but still represents very fine details as demonstrated in this cropped time-lapse sequence from a large FOV image. Objective: CFI Plan Apochromat λ60x oil / NA: 1.4. Sample courtesy of: Michael Davidson, National High Magnetic Field Laboratory, Florida State University

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